Gram staining gram staining is most widely used differential staining in microbiology. Hans christian gram born in 1853 studied botany at the university of copenhagen in. Giemsa stain is commonly used when there is need to examine the blood smear for the parasites but is a good stain for routine examination of blood smear and used to differentiate nuclear and cytoplasmic morphology of the various cells of the blood like platelets, rbcs giemsa staining technique. The name comes from the danish bacteriologist hans christian gram, who developed the technique gram staining differentiates bacteria by the chemical and physical properties of their cell walls. As a result, variables that could cause incorrect test results may be detected and corrected. In bacteriology, the gram staining allows a fast differentiation of bacteria in grampositive and gramnegative. Gram staining of bacteria from environmental sources. The gram stain allows the determination of morphology, dividing bacteria into tw. Gram stain or gram staining, also called grams method, is a method of staining used to distinguish and classify bacterial species into two large groups.
For microbiologists the most fundamental stain was developed in 1884 by the danish bacteriologist hans christian gram. Mechanism of gram variability in select bacteria pdf. Gram staining is a quick procedure used to look for the presence of bacteria in tissue samples and to characterise bacteria as grampositive or gramnegative, based on the chemical and physical properties of their cell. Christian gram 1853 1938, qui a mis au point cette technique en 1882 et qui l a. The mureine structure of the bacteria wall is the basis of the color affinity. Principle, procedure and results february 2, 2015 acharya tankeshwar bacteriology, staining techniques in microbiology 45 gram staining method, the most important procedure in microbiology, was developed by danish physician hans christian gram in 1884. Flood slide with crystal or gentian violet 60 seconds. The gram stain is a classical and important staining technique that remains widely used by. Ziehlneelsen zn method of acid fast staining technique is used to stain mycobacterium species including m. Protocols for using the gram stains in the microbiology laboratory. Bacteria classification by gram staining free essay. Bacteriologist christian, gram positive and gram negative by a staining technique where the ability to avoid decoloration of crystal violet solution by alcohol would render the category of gram positive, and gram negative if the bacteria is decoloured. Gram staining differentiates the bacteria into 2 groups. Verification des performances dune methode selon le sh.